Dr. Melissa Cavallin Johnson
Human Physiology and Neuroscience
Ph.D. in Physiology from Medical College of Georgia, Augusta, GA
B.A. in Biology (minor: Chemistry) from LaGrange College, LaGrange, GA
My research interest is exploring the intercellular and intracellular signaling cascades involved in the chemical senses (taste and smell). I am interested in understanding how ion channels are regulated and how this affects downstream signaling molecules. A voltage-gated potassium channel (Kv1.3) is important for regulating the firing of action potentials in mitral cell neurons of the olfactory bulb. The mitral cell neuron is the primary output neuron of the olfactory bulb that transmits olfactory information to the brain. Altering the activity of Kv1.3 will ultimately change how the brain perceives olfactory sensory information, or in other words, how the brain determines what odors you smell in the air. Tyrosine kinases are enzymes that add a phosphate group to the amino acid tyrosine in proteins. Phosphorylating Kv1.3 closes the channel and decreases K+ current flowing out of the mitral cell. This means that the mitral cell is more excitable because the resting membrane potential is less negative. I am interested in exploring the modulation of Kv1.3 by the tyrosine phosphatase SHP-2, an enzyme that removes phosphate groups from tyrosines in proteins. This reverse reaction may also be important in regulating the firing of action potentials by mitral cell neurons because it would increase K+ current flowing out of the mitral cell making the mitral cell less excitable. Patch-clamp electrophysiology, protein biochemistry, and histology are just a few of the techniques that will be utilized to understand phosphatase regulation of Kv1.3.
BIOL 3513/6513 Human Physiology
BIOL 3526/6526 Vertebrate Histology
BIOL 3134 Cell and Molecular Biology
BIOL 6984 Graduate Seminar
1. Cavallin, M.A. and L.P. McCluskey. 2005. Lipopolysaccharide-induced up-regulation of activated macrophages in the degenerating taste system. J. Neurosci. Res. 80: 75-84. (Figure selected as cover illustration).
2. Cavallin, M.A. and L.P. McCluskey. 2007a. Upregulation of intracellular adhesion molecule (ICAM)-1 and vascular adhesion molecule (VCAM)-1 after unilateral nerve injury in the peripheral taste system. J. Neurosci. Res. 85: 364-372.
3. Cavallin, M.A. and L.P. McCluskey. 2007b. Upregulation of the chemokine monocyte chemoattractant protein-1 following unilateral nerve injury in the peripheral taste system. Neurosci. Lett. 413: 187-190.
4. Colley, B.S.*, M.A. Cavallin*, K.C. Biju, and D.A. Fadool. 2009. Brain-derived neurotrophic factor modulation of Kv1.3 channel is disregulated by adaptor proteins Grb10 and nShc. BMC Neurosci. 10: 8. (*These two authors contributed equally to this publication.)
5. Marks, D.R., K. Tucker, M.A. Cavallin, T.G. Mast, and D.A. Fadool. 2009. Awake intranasal insulin delivery modifies protein complexes and alters memory, anxiety, and olfactory behaviors. J. Neurosci. 29: 6734-6751.
6. Cavallin, M.A., K. Powell, K.C. Biju, and D.A. Fadool. 2010. State-dependent sculpting of olfactory sensory neurons is attributed to sensory enrichment, odor deprivation, and aging. Neurosci. Lett. 483:90-95.
7. Tucker, K., M.A. Cavallin, P. Jean-Baptiste, K.C. Biju, J.M. Overton, P. Pedarzani, and D.A. Fadool. 2010. The olfactory bulb: a metabolic sensor of brain insulin and glucose concentrations via a voltage-gated potassium channel. Results Probl. Cell Differ. 52:147-157.
8. Johnson, M.C., K.C. Biju, and D.A. Fadool. 2011. Sensory enrichment –state dependent sculpting of olfactory bulb mitral cells. (In preparation.)
9. Johnson, M.C., K. Powell, and D.A. Fadool. 2011. Sculpting of olfactory sensory neurons by moderately high fat diet. (In preparation).
1. Jean-Baptiste P., Powell K., Cavallin M., and Fadool D. 2009. Sensory environment affects the abundance of olfactory sensory neurons in the main olfactory epithelium of mice. TriBeta Undergraduate Research Poster Competition at FSU.
2. Tucker K.R., Cavallin M.A., Overton J.M., and Fadool D.A. 2009. Resistance to obesity following Kv1.3-gene targeted deletion is inhibited by olfactory bulbectomy. AChemS XXXI, Abstract P78, Sarasota, FL. Chemical Senses 34: A45.
3. Cavallin M.A., Powell K., Biju K.C., and Fadool D.A. 2009. Sculpting of odorant receptor-identified sensory neurons by odor enrichment/deprivation, aging, and diet. Southeastern Nerve Net (SENN), Jacksonville, FL.
4. Tucker K., Cavallin M., Overton J.M., and Fadool D.A. 2009. Diet-induced obesity resistance of the Kv1.3-null mouse is inhibited by olfactory bulbectomy. Society for Neuroscience (SfN), Chicago, IL.
5. Tucker K., Cavallin M., Jean-Baptiste P., Overton J.M., and Fadool D.A. 2010. A potassium channel expressed in the olfactory bulb subserves as a metabolic sensor. Southeastern Nerve Net (SENN), Atlanta, GA.
6. Fadool D.A., Tucker K., Marks D.M., Cavallin M.A., Overton J.M., and Pedarzani P.. 2010. The olfactory bulb as a metabolic sensor via insulin modulation. AChemS XXXII, Symposium, St. Petersburg, FL.
7. Tucker K.R., Cavallin M., Jean-Baptiste P., Overton J.M., and Fadool D.A. 2010. Ion channel in the olfactory bulb subserves as a metabolic sensor. AChemS XXXII, Poster, St. Petersburg, FL.
8. Cano C.A., Brew T.L., and Cavallin M.A. 2011. Src homology-2 phosphatase expression in mouse olfactory bulb and epithelium. Research Day and Big Night, University of West Georgia, Carrollton, GA.
Underline indicates undergraduate and graduate students that I have supervised.
1. AREA R15 Application (NIH). Grant Number: 1R15DC011923. Phosphatase regulation of olfactory bulb mitral cell activity. (2011-2014).
2. Student Research Assistant Program (UWG), Phosphatase expression in the Olfactory Epithelium and Olfactory Bulb (2010-present).
3. COSM Research Initiative Award (UWG), Phosphatase expression in the Olfactory Bulb and Olfactory Epithelium (2011-2012).