John E. Hansen

 

Curriculum Vitae

(Revised on November 23, 2005)

 

 

I.  Teaching

 

Teaching Appointments

Date                                        Title                            Institution and Department

1985 - 1990                 Teaching Assistant             University of Chicago, Dept. of

        Chemistry, Chicago, IL

1995 - 1996                 Adjunct Professor                University of Detroit, Dept. of

                                                                               Chemistry, Detroit, MI

1996                            Adjunct Professor                Wayne State University,

       Medical School, Detroit, MI

1996 – 2000                Assistant Professor              State University of West Georgia,

                                                                              Dept. of Chemistry, Carrollton, GA

2001 – present             Associate Professor            University of West Georgia,

                                                                              Dept. of Chemistry, Carrollton, GA

 

Courses Taught

COURSE                                                                    YEARS  TAUGHT    

Introductory Chemistry

CHM 111                                                                    Spring 1998

General Chemistry

CHM 122                                                                    Fall 1997

CHM 123                                                                    Winter 1998

CHM 1211                                                                  Fall 1999

CHM 1212K                                                               Summer 2002

Quantitative Analysis

CHM 382                                                                    Winter 1997

 

Analytical Chemistry

CHM 3310K                                                               Fall 1998,  Spring 2000, Fall 2000,

Summer 2001, Fall 2001, Fall 2002,

Summer 2003, Fall 2003, Summer 2004,

Summer 2005

Biochemistry

CHM 471                                                                    Fall 1996,  Fall 1997

CHM 472                                                                    Winter 1997,  Winter 1998

CHM 4711                                                                  Fall 1998,  Fall 1999, Fall 2005

CHM 4712                                                                  Spring 2001, Spring 2003,  Spring 2005

Biochemistry Laboratory

CHM 4720L                                                                Winter 1998,  Spring 2000, Spring 2002

Quantum Chemistry

CHM 3521                                                                  Fall 2001, Fall 2002, Fall 2003,

Fall 2004, Fall 2005

Instrumental Analysis

CHM 483                                                                    Spring 1997,  Spring 1998

CHM 4330                                                                  Spring 1999, Spring 2000, Spring 2001,

Spring 2002, Spring 2003, Spring 2004,    Spring 2005

Inorganic Chemistry

CHM 4610                                                                  Spring 2000

Advanced Laboratories

CHM 4911L                                                                Spring 1999, Spring 2001, Spring 2003

                                                                                    Spring 2004

CHM 4912L                                                                Fall 1999,  Fall 2000, Fall 2001,

Fall 2002, Fall 2003, Fall 2004,

Fall 2005

Environmental Science

ENVS 1202                                                                 Spring 1997

XIDS 2202                                                                  Spring 2003

Science Foundation

XIDS 2201                                                                  Fall 1999,  Fall 2000

Seminar Courses

Sophmore Seminar                                                       Spring 2004, Spring 2005

Senior Seminar                                                 Spring 2002, Spring 2005

 

Guest Lecturer

Course Number                                  Year                                        Topic

Spectroscopy in                                    1997                Nuclear Magnetic Resonance Theory,

Organic Chemistry                                                        (a one week presentation)

CHM 490

 

Spectroscopy in                                    1998                Nuclear Magnetic Resonance Theory,

Organic Chemistry                                                        (a one week presentation)

CHM 490

 

Physical Chemistry II                            1998                Fourier Transform Theory        

CHM 442                                                                    (a half week presentation)

 

Advanced Topics in                              1999                Molecular Orbital Theory         

Organic Chemistry                                                        (a one week presentation)

CHM 4485

 

Chemical Kinetics                                 2000                Enzyme Kinetics

CHM 4930                                                                  (a three week presentation)

Spring 2001

MCAT Review Course:  reviewed Math, Electronics and Electrochemistry over a couple evening classes.

 

Fall 2001

GRE Review Course: reviewed Analytical Chemistry over a couple evening classes.

 

Spring 2002

MCAT Review Course:  reviewed Math, Electronics and Electrochemistry over a couple evening classes.

Spring 2003

MCAT Review Course:  reviewed Math, Electronics and Electrochemistry over a couple evening classes.

 

 

Program Development           In June 2004, the American Chemical Society certified the Biochemistry Degree Program that I had designed and developed between the years 1998 and 2001.  This degree program allows students to obtain a BS degree in chemistry with a specialized emphasis in biochemistry.  This required the redesign of the Biochemistry Course (CHM 4711) in the Fall of 1999, development of the Biochemistry Lab Course (CHM 4720L) in the Winter of 1998, and the development of the Physical Biochemistry Course in the Spring of 2001.

 

Course Development

Analytical Chemistry             I developed a complete set of power point notes to be

CHM 3310K                           used for a studio format.  I also developed a number of the experiments to be used in this format.

 

Instrumental Analysis            I wrote a detailed set of class notes to be used for this

CHM 4330                              course. 

 

Biochemistry                          Dr. Geisler and I, during semester conversion, redesigned

CHM 4711                              the previous two-quarter course in biochemistry into a one-semester course.  I developed a complete detailed set of class notes for this course.

 

Physical Biochemistry          In the spring of 2001, I developed and designed a new course  CHM 4712                                that  covered topics in the physical biochemistry and

spectroscopy of biological macromolecules.  I developed a complete detailed set of class notes for this course.

 

Biochemsitry Laboratory      In the Winter of 1998,I developed a new laboratory course that

CHM 4720L                            has two  objectives.  The first is to introduce the student to the practices of protein separation, purification, and quantification.  The other objective is to cover the principles of physical biochemistry, which focus on the properties of biological macromolecules.  Many of the laboratory procedures employ instrumentation purchased through the successful acquisition of a NSF-ILI grant, in which I was the principle investigator.

 

Inorganic Chemistry              In Spring of 2000, I designed and developed a new course

CHM 4610                              in bioinorganic chemistry, and wrote a set of class notes for it.

 

Advanced Laboratory I         Along with Dr. Kahn, I helped to develop part I of an

CHM 4911L                            integrativelaboratory course, which emphasize principles of quantum chemistry, thermodynamics, kinetics and instrumental analysis.  I developed a number of new labs for this course. Many of the laboratory procedures employ instrumentation purchased through the successful acquisition of two NSF-ILI grants.  One of those grants I was the principle investigator, and the other grant I was the co-investigator with Dr. Leavitt, and Dr. Khan was the principle investigator.

 

Advanced Laboratory II        Along with Dr. Leavitt, I helped develop part two of an

CHM 4912L                            integrative laboratory course, which emphasizes principles of quantum chemistry, thermodynamics, kinetics and instrumental analysis.  Laboratory procedures employ instrumentation purchased through the successful acquisition of two NSF-ILI grants (discussed above).

WAC Courses Taught (I have taught 16 WAC courses over 6 years)

WAC Courses Taught                       Year

CHM 3310K                                       Fall 1998

CHM 4471                                          Fall 1998, Fall 1999, Fall 2005

CHM 4911L                                        Spring 1999, Spring 2001, Spring 2003, Spring 2004,

                                                            Spring 2005

CHM 4912L                                        Fall 1999,  Fall 2000, Fall 2001, Fall 2002, Fall 2003,

                                                            Fall 2004, Fall 2005

 

Undergraduate Research Students Supervised

Student Presentations at the National Collegiate Honors Council  (Student research carried out in my lab and that I directed):

 

1.      Yong SuhThe Intricate World of Proteins,  presented at the National Collegiate Honors  Council 34th Annual Conference, Orlando, FL, October 5-10, 1999

2.      Mary Kate McBrayer, Azurin: Stability of a Protein Structure, presented at the

National Collegiate Honors Council 35th Annual Conference, Washington, D.C., 

October 18- 122, 2000.

3.      Christopher D. Lane, Velocity of Sound in a Liquid, presented at the National

Collegiate Honors Council 35th Annual Conference, Washington, D.C., October 18-

122, 2000.

4.  Toma Omonuwa, The Naked Electron: Photoexcitation of N-Acetyl Tryptophan.   

       National Collegiate Honors Council (NCHC) meeting, Chicago, Nov 5-9, 2003

5.      Laura Altom, The Promiscuous Behavior of a Molecular Chaperone. National Collegiate Honors Council (NCHC) meeting, Chicago, Nov 5-9, 2003.

6.      Patricia Onuegbu, Effect of Age-dependent modification of alpha-crystallin on the chaperone function. National Collegiate Honors Council (NCHC) meeting, Chicago, Nov 5-9, 2003.

7.   Logan Leslie,  Unfrying an Egg. National Collegiate Honors Council (NCHC) meeting,

      Chicago, Nov, 2004.

8.   Logan Leslie,  Chunnel Disaster. National Collegiate Honors Council (NCHC) meeting,

      Chicago, Nov, 2005.

 

Student Presentations at the National Conference on Undergraduate Research  (Student research carried out in my lab and that I directed):

 

1.      Patricia Oneugbu, J.E. Hansen and S. Swamy-Mruthinti. Effect of Age-dependent modifications of a-crystallin on its chaperone function. National Council of Undergraduate Research, NCUR-2004, Indianapolis, IN, April, 15-17, 2004.

2.      Logan Leslie, and John E. Hansen.  Can We Unfry the Egg?: The process of protein misfolding.  National Council of Undergraduate Research, NCUR-2005, Indianapolis, IN, April, 21-23, 2005.

 

Student Presentations at Regional and Local Meetings

 

1.      James Burgess and John Hansen,  Trapping Intermediates in the Spontaneous and

GroEL Assisted Refolding of the Bacterial Protein Glucose-6-Phosphate Dehydrogenase, presented at the 1997 Georgia Academy of Science Meeting.

2.      Michael Robbins, Bryan Green and John E. Hansen,  GroEl Assists in the Refolding

of a Bacterial Protein that is Kinetically Trapped in a Misfolded State without Aid of

GroES, presented at the 1998 Sigma Xi Student Research Presentations.

3.      Bryan Green, Michael Robbins and John E. Hansen,  GroES inhibits the GroEL-

assisted Refolding of Glucose-6-phosphate Dehydrogenase at Low Temperatures, presented at the 1998 Southeast Regional Meeting of the American Chemical Society, November 4-7, 1998.

4.      Yong Suh, Mary Kate McBryer, Michael Robbins and John E. Hansen,  An

Examination of Factors that Affect Stability and Folding of a b-Sheet Protein, presented at the 1998 Sigma Xi Student Research Presentations.

5.      Mary Kate McBrayer, Michael Robbins, Yong Suh and John E. Hansen,  The Role of a

Disulfide Bond in Affecting the Stability of a b-sheet Protein, presented at the 1998 Sigma Xi Student Research Presentations.

6.      Christopher D. Lane, David J. Hathcock, Andrew J. Leavitt, John E. Hansen and

Farooq A. Khan,  Measurement of the Velocity of Sound in Liquids via Pump-probe Laser Spectroscopy,  presented at the 1999 Southeastern Regional Meeting of the American Chemical Society,  October 17-20.

7.      Mary Kate McBrayer, Yong Suh , Michael Robbins, and John E. Hansen,  The

Effect of pH and Self-Association on the Unfolding Pathway of the Protein Azurin, presented at the 1999 Southeast Regional Meeting of the American Chemical Society, October 17-20.

8.      Mary Kate McBrayer, Yong Suh and John E. Hansen, The Unfolding and Refolding

of Apoazurin, , presented at the 1999 Southeast Regional Meeting of the American Chemical Society, October 17-20.

9.      Yong Suh, Mary Kate McBrayer and John E. Hansen,  The Thermodynamics and

Kinetics of Refolding of a Metalloprotein, presented at the 2000 Sigma Xi Student Research Presentations.

10.  Christopher L. Lane, David J. Hathcock, Andrew J. Leavitt, John E. Hansen, Farooq

A.     Khan, Department of Chemistry,  Measurement of the Velocity of Sound in a Liquid Via Laser Spectroscopy, presented at the 2000 Sigma Xi Student Research Presentations.

11. Mary Kate McBrayer and John E. Hansen, The Folding and Unfolding of Zn(II) Azurin, presented at the 2001 Sigma Xi Student Research Presentations.

12. Mary Kate McBrayer, Mad Hatters, Mad Cows and Bad Protein, talk presented at the State University of West Georgia's Big Night, March 2001.

13.  Justin Russell, John Hansen and S. Swamy-Mruthinti. Structure-function relationship of AQP0 in the Human eye lens. Sigma Xi Student presentations, University of West Georgia, Carrollton, GA, March, 2002.

14.  Kristin Coker, John Hansen and S. Swamy-Mruthinti, Cataract formation in lenses of Diabetic Rats. Sigma Xi Student presentations, University of West Georgia, Carrollton, GA, March, 2002.

15. Justin Russell, John Hansen and S. Swamy-Mruthinti, Structure of AQP0 in Human

      Eye lenses, presented at ARCH, State University of West Georgia, April, 2002.

16.  Kristin Cooker, John Hansen and S. Swamy-Mruthinti. Expression and characterization of lysine mutants of AQP0 in baculovirus expression system, Presented at ARCH, University of West Georgia, Carrollton, GA, April, 2002.

17.  Angela Kersh, S. Swamy-Mruthinti and John E. Hansen. Specificity of alpha crystallin gene products in preventing thermal aggregation of AQP0. Sigma XI Student presentations, University of West Georgia, Carrollton, GA, March 2003

18.  Patricia Onuegbu, John Hansen, and S. Swamy-Mruthinti. Effect of age-dependent modifications of alpha-crystallin on its chaperone function. Sigma Xi student presentations, University of West Georgia, Carrollton, GA, March 2003.

19.  Laura Altom, S. Swamy-Mruthinti and John E. Hansen. Developing a functional assay to determine the structural integrity of AQP0 following thermal stress. Sigma Xi Student research competitions, University of West Georgia, Carrollton, GA, March 2003

20.  Angela Kersh, John Hansen and S. Swamy-Mruthinti. Immunological characterization of Aquaporin ) and alpha-crystallin interaction.  9th Annual Student conference for Research and Creative Arts. April, 2003, University of Houston-Clear Lake, Houston, TX.

21.  Patricia Onuegbu, J.E. Hansen and S. Swamy-Mruthinti. Effect of Age-dependent modification of alpha-crystallin on the chaperone function. National collegiate Honors Council (NCHC) meeting, Chicago, Nov 5-9, 2003

22.  Patricia Oneugbu, J.E. Hansen and S. Swamy-Mruthinti. Effect of Age-dependent modifications of a-crystallin on its chaperone function. National Council of Undergraduate Research, NCUR-2004, Indianapolis, IN, April, 15-17, 2004

23.  Joseph Fachini, J.E. Hansen and S. Swamy-Mruthinti, Interaction of AQP0 and alpha crystallin during thermal stress. 65th Annual meeting of Southeastern Biologists, April 14-17, Memphis, TN

24.  Logan Leslie, Cathy Tran, J. E. Hansen and S. Swamy-Mruthinti. A study into the aggregation of membrane proteins. Sigma Xi Student research competitions, University of West Georgia, Carrollton, GA, March, 2004

25.  Joseph Fachini, S. Swamy-Mruthinti and J.E. Hansen. Immunochemical characterization of Aquaporin and alpha crystallin interaction during thermal stress. Sigma Xi Student research competitions, University of West Georgia, Carrollton, GA, March, 2004.

26.  Patricia Onuegbu, S. Swamy-Mruthinti and J.E. Hansen. Does age-dependent loss of protective mechanisms exacerbate protein denaturing? Sigma Xi Student research competitions, University of West Georgia, Carrollton, GA, March, 2004

27. Toma Omonuwa, Farooq Khan, and John Hansen. Light Induced Electron Transfer from Tryptophan.  Sigma XI Student presentations, State University of West Georgia, Carrollton, GA, March 2004.

28.  Logan Leslie, Cathy Tran, S. Swamy-Mruthinti, and John Hansen.  A Study into the  

      Aggregation of a Membrane Protein.  Big Night, State University of West Georgia, April

      2004.

29. Toma Omonuwa, Farooq Khan, and John Hansen. Light Induced Electron Transfer

       from Tryptophan.  Big Night, State University of West Georgia, Carrollton, GA, April

       2004.

30. Patricia Onuegbu, John Hansen and S. Swamy-Mruthinti.  Does Age Dependent

      Loss of Protective Mechanisms Exacerbate Protein Denaturation.  Big Night, State

      University of West Georgia, April 2004.

31. Logan Leslie, S. Swamy-Mruthinti, and John E. Hansen. London Bridge is Falling Down: the unraveling of a membrane protein under thermal stress.  Sigma XI Student Presentations, State University of West Georgia, Carrollton, GA, March 2005.

32. Logan Leslie, S. Swamy-Mruthinti, and John E. Hansen Determining the Stability of a Membrane Protein.  Big Night, State University of West Georgia, Carrollton, GA, April, 2004.

 

Undergraduate student publications:

 

1.      Patricia Onuegbu, (Advisor: S. Swamy-Mruthinti and Co-Advisor: J. E. Hansen). Effect of age-dependent modifications of a-crystallin on its chaperone function. Proceedings of the NCUR-2004.

 

Undergraduate Research Students

James Burke                             Justin Russell

Amanda Harris             Sarah Morgen

Moses Langford                       Kristin Cocker

Michael Robbins                       Toma Omonuwa

Bryan Green,                            Luara Altom

Mary Kate McBrayer               Patricia Onuegbu

Christopher Lane                      Jonathon Meyers

Clinton Shelley              Logan Leslie

Yong Suh                                 Cathy Tran

Eric Hall                                   Ben Browning

Chelsea Dixon                          Chris Shacklady

 

Notable Accomplishments of Undergraduate Research Students that I Mentored

Among the research students I have mentored, three have won the Goldwater Scholarship, and one has won the Marshall Scholarship.

 

Michael Robbins

-    Accepted into Loma Linda Medical School in the Fall of 2000.

Yong Suh

-         In 2002, Yong began two years of graduate studies at Oxford University.

-         Recipient of the Marshall Scholarship in the year 2001.

-         In 2001, Yong took a one year internship to work directly under Dr. Francis Collins, Director of the Human Genome Project.

-         Recipient of the Goldwater Scholarship in the year 2000.

-         Accepted into the highly competitive Summer Research Program at the National Institutes of Health in Bethesda MD. 

-         Presented research at the 34th and 35th meetings of the National Collegiate Honors Council.

-         Received second place awards at both the 1999 and 2000 Sigma Xi Student Research Presentations.

Christopher Lane

-    Accepted into graduate school at Stanford, Berkeley, Columbia and Georgia Tech.  He

      decided to attend Georgia Tech.

-    Accepted into the REU program at Georgia Institute of Technology during the 

 summer of  2000.  Worked for the internationally-renowned laser spectroscopist Mostafa  

 El-Sayed.

-    Presented research at the 35th meeting of the National Collegiate Honors Council.

Mary Kate McBrayer

-    Accepted into graduate school at MIT, Wisconsin, and Cornell.  She decided to attend

     Cornell.

-    During the Summer of 2001, Mary Kate participated in an REU at MIT.  She worked in

     the laboratories of Professor Jonathon King

-    In March 2001, Mary Kate won the Sigma Xi Paper Competition

-    Presented research at the 35th meeting of the National Collegiate Honors Council.

 

Laura Altom

-         Accepted into medical school at Yale, University of Michigan, and Vanderbilt.  She 

      accepted a full scholarship to attend Vanderbilt Medical School.

-         Presented her research at the 38th meeting of the National Collegiate Honors Council.

 

Toma Omonuwa

-         Accepted into medical school at Johns Hopkins, Yale, and Duke.  She accepted a full

      scholarship to attend Duke Medical School.

-    In 2003, Toma was the recipient of the Goldwater Scholarship.

-    Presented her research at the 38th meeting of the National Collegiate Honors Council.

 

Leslie Logan

-         In 2005, Logan was recipient of the Goldwater Scholarship.

-         During the Summer of 2005, he carried out research in the laboratories of Professor Richard Zare at Stanford University.

-         Presented his research at the 39th meeting of the National Collegiate Honors Council.

-         Presented his research at the 40th meeting of the National Collegiate Honors Council.

 

 

 

 

 

 

 

 

 

 

 

 

 

II.  Service to the Institution

 

Committee Membership

Departmental Level

1997 - 2005    Library Liaison.

1998                Participated in semester conversion.

1997 – 2001    Designed and developed biochemistry program.

1998                On Biology Faculty Search Committee, reviewing candidates for two faculty

                        positions.

2001 – 2002   WAC representative.

2003                Headed up Dr. Khan’s post tenure review.

2003 – 2005   Faculty advisor to the ACS Club.

2004 – 2005   Volunteer advisor at the Freshmen Center.

 

Committees of the College of Arts and Sciences

1999– 2000     Member of the Faculty Advisory Committee.

2002 – 2003    Member of the Tenure and Promotion Committee.

 

University Wide Committees

1996                Nominated charter member of team that participated in the Council for Undergraduate Research (CUR) meeting, Ashville, NC, November 1996.

1996 – 1997    Participated in the early meetings that led to the Committee for Celebration of Scholarship and Creative Activities, 1996 - 1997.

1999                Participated in the Mentoring Program for New Faculty.

2001 – 2004    Member of the Faculty Senate.

2001                Member of the General University Matters Committee.

2002 – 2004    Chair of the General University Matters Committee.

2002 – 2004    Member of the University Club Steering Committee.

2002 – 2004    Member of the Parking Subcommittee.

2003 – 2004    Chair of the Master Planning Subcommittee on transportation and parking.

2005 – 2007    Member of the Learning Resource Committee.

Academic Advising

1.   B.S. majors with biochemistry emphasis.

2.   B.S. majors.

3.   Pre-pharmacy.

4.  Advisor at Freshmen Center (2004 – 2005).

 

Other Service to the University

2000– 2002     Vice President of Sigma Xi

2003 – 2004    President of Sigma Xi

 

Other Service to the Department

1.      Set up meeting "Food for Thought" to introduce perspective majors to Chemistry Dept.

1.      Arranged for Professor El Sayed’s Visit and Talk.

2.      Took over Dr. Esslinger’s Forensics Class during last several weeks of the fall 1998 semester, while Glenn was recuperating from surgery.

3.      Arranged for Professor Robbin's Visit and Talk.

4.      Directed two REU projects during the summer of 1999.  Mentored four students, which 

required a commitment of time over 40 hours per week for ten weeks.

 

Participation in Educational Activities

Educational Outreach

1.      Coordinator of an event in the High School Science Olympiad (1997, 1998, 1999, 2000).

2.      Reader in the High School Science Bowl (1997, 1998, 1999).

3.      Took part in Science Saturday, an event to attract high school students to major in chemistry at UWG, 1998.

4.      Participated in the PREP program for junior high school students, 1999.

5.       Visited Fayetteville High School with Dr. Garmon, 1999.

6.      Visited and gave a chemistry demonstration at Carrollton Elementary School, 2003.

7.      As faculty advisor for the ACS club, I have helped the students with coordinating mole day.  An opportunity where the ACS club invites local high school students to our department for a visit and a chemistry demonstration (2004, 2005).

8.      In the Fall of 2005,  I began a website called UWG MarsWatch.  Following the 2005 Mars apparition, the name of the site will change to UWG SkyWatch.  Its emphasis will be broaden to general astronomical interests.

 

External Activities

1.      Panelist - Reviewer of NSF ILI Grants, January 28 - 31, 1998.

2.      Panelist - Reviewer of NSF CCLI Grants, August 2-5, 1999.

3.   I have reviewed a total of twelve papers for the following journals:

 

Biophysica Biochemica Acta

Chemical Physics Letters

The Chemical Educator

 

Participation in Community Activities

2000 - 2001     A member of the Carrolton School's Gifted Education Advisory Council.

 

2002 – 2005    A USA Swimming Official.  I volunteer 250+ hours per year officiating at local and away swim meets.

                        USA Swimming Certifications:

January 2002:   Certified as a Stroke & Turn Judge for the Georgia LSC.

June 2004:          Certified as a Starter for the Georgia LSC.

February 2005:  Certified as a Stroke & Turn Judge at the national level.

August 2005:      Certified as a Starter at the national level.

At the present time there are only two certified starters in the western half of the state, and I am the only nationally certified starter in West Georgia.

 

2005                I received the “Bronze Level Award’ from Georgia Swimming for having officiated at 100 sessions.  Each session is on average 5 hours long.

 

2005                I was awarded  “Volunteer of the Year” by the Carrollton Parks, Recreation and Cultural Arts Department at their annual banquet for volunteers.

 

2005 – 2007    A member of the board for the Carrollton Bluefin Swimming Organization.

III.   Academic Achievement

 

Degree and Discipline                                   School                                     Years

B.S.                                         University of Wisconsin, Madison, WI  1973 - 1980

Two Majors:  Chemistry & Mathematics

 

Ph.D.                                       University of Chicago, Chicago, IL                    1985 - 1991

Discipline:  Physical Chemistry            Dissertation:  Ultrafast Photophysics of Proteins

 

Postdoctoral Fellow                University of Michigan, Ann Arbor, MI 1991 - 1994

Discipline:  Biophysics

 

Honors

1.      Astronomical discovery recorded in the Journal of the Association of Lunar and Planetary Observers (July 1976, p. 227).  I was part of a small group of amateur astronomers that discovered the first known atmospheric disturbance in the Northern Hemisphere of Jupiter employing the 15.5-inch Clark refracting telescope at the University of Wisconsin's Washburn Observatory.

 

2.      I was part of a small group of students to win the First Place Award for "Research and Development" at the 1977 University of Wisconsin Engineering Exposition.  Our research, in the area of biomechanics, centered on developing a therapeutic means of enervating atrophied muscle.  Over several hundred student entries were exhibited.

 

3.      The paper Photophysics of Metalloazurins (Biochemistry 29, 7329) was specially highlighted in the journal "Current Opinion in Structural Biology" (1991, page 1058) as a paper of Outstanding Experimental Work.   This journal is a review of all advances and an evaluation of key references in the area of structural biology.

 

4.      I was a recipient of a two-year NIH Traineeship Award.  The award beginning in 1992 totaled $40,000.

 

5.   Invited to present my research at the 1993 Gordon Research Conference on

Proteins, Tildon, NH, June 13 - 18.

 

6.      Recipient of the Pharmacia Biotech Award in the amount of $400 for research that I presented at the Seventh Symposium of the Protein Society, San Diego, July 24 -28, 1993.

 

7.      Invited by the Editor in Chief of the journal "Cell Biochemistry and Biophysics" to write a review paper on "protein folding". 

 

 

 

IV.              Professional Growth and Development

 

Membership in Professional Societies

American Chemical Society

American Institute of Physics

Federation of American Societies of Experimental Biology

Biophysical Society

Optical Society of America

American Association of Variable Star Observers

Sigma Xi

 

Peer-reviewed Publications

1.      John E. Hansen, Timothy Sharpe, and E. Edward Bittar (1986) Phosphate Metabolites in Single Barnacle Muscle Fibers Investigated by Phosphorous-31 Nuclear Magnetic Resonance, Comp. Biochem. Physiol., B: Comp. Biochem. 83, 875.

2.      John E. Hansen, James W. Longworth, and Graham R. Fleming (1990) Photophysics of Metalloazurins, Biochemistry 29, 7329.

3.      John E. Hansen, Sandra J. Rosenthal, and Graham R. Fleming (1990) Subpicosecond Fluorescence Anisotropy Measurements of Tryptophanyl Residues in Proteins, in Ultrafast Phenomena VII (Harris, C.B., Ippen, E.P., Mourou, G.A., and Zewail, A.H., Eds.) Springer-Verlag, Berlin.

4.      John E. Hansen, Sandra J. Rosenthal, and Graham R. Fleming (1992) Subpicosecond Fluorescence Depolarization Studies of Tryptophan and Tryptophanyl Residues of Proteins, J. Phys. Chem. 96, 3034.

5.   John E. Hansen, Ehud Pines, and Graham R. Fleming (1992) Excited-State Proton

Transfer of Protonated  1-Aminopyrene Complexed with SYMBOL 98 \f "Symbol"-Cyclodextrin, J. Phys. Chem. 96, 6904.

6.   John E. Hansen and Ari Gafni (1993) Thermal Switching between Enhanced and

Arrested Reactivation of Bacterial Glucose-6-Phosphate Dehydrogenase Assisted by GroEL in Absence of ATP, J. Biol. Chem.  268, 21632.

7.   John E. Hansen and Ari Gafni (1994) Fluorescence Detection of Conformational

Changes in GroEL Induced by Thermal Switching and Nucleotide Binding, J. Biol.

Chem. 269, 6286.

8.  John E. Hansen, Duncan G. Steel, and Ari Gafni (1996) Detection of a pH

Dependent Conformational Change by Room-Temperature Time-Resolved Phosphorescence in Apo and Metal Substituted Derivatives of Azurin, Biophysical J. 71, 2138.

9.   Farooq Khan and John E. Hansen (2000) The Dirac (Bra-Ket) Notation in the

Undergraduate Physical Chemistry Curriculum: A Pictorial Introduction,  The Chemical Educator 5, 113.

10. Chrsitopher Lane, David Hathcock, Andrew Leavitt, Farooq Kahn, and John E.

Hansen, Measuring the Compressibility of a Liquid by a Pump-probe Laser Technique, The Chemical Educator (2001) 6, 235 - 237.

11. John E. Hansen, Michael Robbins, Mary Kate McBrayer, and Yong Suh, The

Thermodynamics and Kinetics of the Unfolding and Refolding of Apoazurin and Zn(II) Azurin, Cell Biochemistry and Biophysics (2002) 36, 19 – 41.

12.  John E. Hansen and S. Swamy-Mruthinti, The Mechanism of Thermal Denaturation of AQP0 in the Absence and Presence of a-crystallin (in preparation).

13.  Swamy-Mruthinti, S., Hansen, J.E., Srinivas, V., Mruthinti, S. and Mohan Rao, Ch. Alpha A Crystalline Protects AQP0 from Thermal Denaturation (in preparation).

 

 

Published Abstracts in Proceedings of National Meetings

1.      John E. Hansen, Timothy Sharpe, and E. Edward Bittar,  31P-NMR Studies of

Metabolite Levels in Single Barnacle Muscle Fibers, at the 35th Annual Fall Meeting of the American Physiological Society, 26 -31 August 1984.

2.      John E. Hansen, James W. Longworth, and Graham R. Fleming,  Long-Range

Electron Transfer in Metalloazurins, at the 16th Annual Meeting of the Society for Photobiology, 13 - 17 March 1988.

3.      John E. Hansen, James W. Longworth, and Graham R. Fleming,  Electronic Energy

Transfer in Metalloazurins, at the SPIE Symposium "Time-Resolved Laser Spectroscopy in Biochemistry II", 15-17 January 1990.

4.      John E. Hansen, Sandra J. Rosenthal, and Graham R. Fleming,  Subpicosecond

Fluorescence Anisotropy Measurements of Tryptophanyl Residues in Proteins, at the 7th Ultrafast Phenomena Meeting, 14-17 May 1990.

5.  John E. Hansen, Duncan G. Steel, and Ari Gafni,  PH Dependence of Time-Resolved

            Room-Temperature Phosphorescence of Apo and Metallazurins, at the 37th Annual Biophysical Meeting, 14-18 February 1993.

6.  John E. Hansen and Ari Gafni,  Thermal Switching of the GroEL-Assisted

Renaturation of Glucose-6-Phosphate Dehydrogenase, at the 1993 Gordon Research Conference on Proteins, June 13-18.

7.      John E. Hansen and Ari Gafni,  Thermal Switching in the Enhanced Reactivation of

Glucose-6-Phosphate Dehydrogenase assisted by GroEL in the absence of GroES and ATP, at the Seventh Symposium of the Protein Society, 25-30 July 1993.

8.      John E. Hansen and Ari Gafni,  Detecting Conformational Changes in GroEL

Induced by Thermal Switching and Nucleotide Binding, at the 38th Annual Biophysical Meeting, 3-8 March 1994.

9.      John E. Hansen and Ari Gafni,  Detection of Conformational Changes in GroEL

Induced by Thermal Switching and Nucleotide Binding and their Correlation to Function, at the 1994 Cold Spring Harbor Meeting on Biology of Heat Shock Proteins and Molecular Chaperones, May 4-8.

 

 

 

10.  John E. Hansen and Ari Gafni,  Pathways in the Spontaneous and GroEL-assisted

Refolding of Glucose-6-phosphate dehydrogenase, at the 39th Annual Biophysical Meeting, 12-16 February 1995.

11.  John E. Hansen and Ari Gafni,  Identifying Pathways in the Spontaneous and

GroEL-assisted Refolding of Glucose-6-phosphate Dehydrogenase by Using GroEL to Trap Folding Intermediates, at the 1995 Keystone Symposia Meeting on Heat Shock Proteins in Biology and Medicine, February 27 - March 5.

12.  John E. Hansen and Michael Robbins,  An Examination of Factors that Affect Stability

and Folding of a b-sheet Protein, presented at the 43rd Annual Meeting of the Biophysical Society, February 13 – 17, 1999.

13. L. Hibbard, S. Edmonds, J. Hansen, and G. Carter,  UV and Electrolyte Effects on the

 Spectral Properties of Alpha- and Beta- Crystallins, presented at the 27 th Annual  

 Meeting of the American Society for Photobiology,  July 10-15, 1999.

14.  Lisa B. Hibbard, Sadiqa Edmonds, Khadela Johnson, and John Hansen,  Effects of

      Salt and Thermal Stress on the Configuration of a-Crystallin, submitted to the    

      International Congress on Photobiology, San Francisco, CA, July 1-7, 2000.

15.  Swamy-Mruthinti, S., Hansen, J.E., Russell J. and Mruthinti, S. Structure-function relationship of AQP0 in human cataract lenses. Invest. Ophthalmol. Vis. Sci, 43, 2002

16.  Swamy-Mruthinti, S., Srinivas, V., Hansen, J.E. and Mohan Rao Ch. Prevention of Thermal aggregation of AQP0 by α crystalline, Exp. Eye Res., 72, (suppl 2), p40, 2002

17.  Hansen, J.E., Srinivas, V., Kersh, A.M., Mohan Rao, Ch. and Swamy-Mruthinti, S. Alpha crystallin prevents thermal aggregation of AQP0. Biophys. J. 84, (suppl 2) 28a, 2002.

18.  S. Mruthinti, J.E. Hansen, A.E. Kersh, V. Srinivas, Ch. Mohan Rao and S. Swamy-Mruthinti. Role of C-terminus of AQP0 during thermal-stress induced interaction with alpha crystallin.  Invest. Ophthalmol. Vis. Sci, 44 (suppl), 2003

19.  S. Swamy-Mruthinti, J.E. Hansen and S. Mruthinti. Interaction of a crystallin with AQP0 during thermal stress. A focus on the lens: Integrating structure, function, differentiation and cataract. The proceedings of ICER 2004 satellite meeting

20.  S. Swamy-Mruthinti, J.E. Hansen and S. Mruthinti. Thermal denaturation of AQP0 and protection by a crystallin. Exp. Eye Res, 79 (suppl), 156, 2004.

 

 

Published Abstracts in Proceedings of Regional Meetings

1.      James Burgess and John Hansen,  Trapping Intermediates in the Spontaneous and

GroEL Assisted Refolding of the Bacterial Protein Glucose-6-Phosphate Dehydrogenase, presented at the 1997 Georgia Academy of Science Meeting.

2.      John Hansen and James Burgess,  Trapping Intermediates During the Refolding of

the Bacterial Protein Glucose-6-Phosphate Dehydrogenase, presented at the 1997 Southeast Regional Meeting of the American Chemical Society, October 19-22, 1997.

3.      John E. Hansen, Michael Robbins, and Bryan Green,  Rescuing a Kinetically

Trapped Misfolded Protein, presented at the 1998 Southeast Regional Meeting of the American Chemical Society, November 4-7, 1998.

4.      Bryan Green, Michael Robbins and John E. Hansen,  GroES inhibits the GroEL-

assisted Refolding of Glucose-6-phosphate Dehydrogenase at Low Temperatures, presented at the 1998 Southeast Regional Meeting of the American Chemical Society, November 4-7, 1998.

5.      Farooq A. Khan, Andrew J. Leavitt, John E. Hansen, David J. Hathcock, and

Christopher L. Lane, Modernization of Undergraduate Curriculum via Laser Based Spectroscopies, presented at the 1998 Southeastern Regional Meeting of the American Chemical Society,  November 4-7.

6.   Khadela Johnson, Sadiqa Edmonds, Lisa Hibbard, and John Hansen, Effect of Salt

on Heat Induced Structural Alterations in a-Crystallin, presented at the Research Infrastructure in Minority Institutions Annual Research Symposium in Atlanta, GA, February 18-21, 1999.

7.   John E. Hansen, Michael Robbins, Mary Kate McBrayer, and Yong Suh,  The Effect

of pH and Self-Association on the Unfolding Pathway of the Protein Azurin, presented at the 1999 Southeast Regional Meeting of the American Chemical Society, October 17-20.

 

8.      Farooq A. Khan, Andrew J. Leavitt, John E. Hansen, David J. Hathcock, and

Christopher L. Lane, Modernization of Undergraduate Education using Laser Based Spectroscopies, presented at the 1999 Southeastern Regional Meeting of the American Chemical Society,  October 17-20.

9.      Christopher D. Lane, David J. Hathcock, Andrew J. Leavitt, John E. Hansen and

Farooq A. Khan,  Measurement of the Velocity of Sound in Liquids via Pump-probe Laser Spectroscopy,  presented at the 1999 Southeastern Regional Meeting of the American Chemical Society,  October 17-20.

10. Mary Kate McBrayer, Yong Suh and John E. Hansen, The Unfolding and Refolding

of Apoazurin, , presented at the 1999 Southeast Regional Meeting of the American Chemical Society, October 17-20.

11. John E. Hansen and Mary Kate McBrayer, The Effects of a Metal Cation on the Folding of a Blue Copper Protein, presented at the 2001 Southeast Regional Meeting of the American Chemical Society, September 23-26.

12. John E. Hansen, Laura Altom and S. Swamy-Mruthinti. Protection of a membrane

     protein during thermal stress by alpha-crystallin SERMACS meeting, Atlanta, Nov 16, 

     2003.

 

Invited Talks

1.      “The Promiscuous Behavior of a Molecular Chaperone”, Chemistry Seminar at

Spelman College, Atlanta, GA, October 9, 1997.

2.      “Some Like it Hotter:  The Role of Negative Temperature in Lasers and Nuclear

Magnetic Resonance”,  Physics Seminar at State University of West Georgia, October 7, 1998.

3.      "On the Rack will a Protein Divuldge its Secrets: A story of a blue copper protein.",

Inorganic Chemistry Seminar Series at the University of Georgia, Athens, GA, March 27, 2000.

4.      "Mad Hatters, Mad Cows, and Bad Protein", Sigma Xi Talk at the State University of

West Georgia, November 3, 2000.

.5.  Helping to Change an Institution’s Culture”,  The Center for Teaching and 

       Learning, The University of Chicago, Chicago, IL, June 1, 2002.

 

Papers Read

1.      John E. Hansen, James W. Longworth, and Graham R. Fleming,  Electronic Energy

Transfer in Metalloazurins, at the SPIE Symposium "Time-Resolved Laser Spectroscopy in Biochemistry II", 15-17 January 1990.

2.      John E. Hansen, Sandra J. Rosenthal, and Graham R. Fleming,  Subpicosecond

Fluorescence Anisotropy Measurements of Tryptophanyl Residues in Proteins, at the 7th Ultrafast Phenomena Meeting, 14-17 May 1990.

3.      John E. Hansen and Ari Gafni,  Pathways in the Spontaneous and GroEL-assisted

Refolding of Glucose-6-phosphate dehydrogenase, at the 39th Annual Biophysical Meeting, 12-16 February 1995.

4.      John E. Hansen, The Promiscuous Behavior of a Molecular Chaperone, Chemistry

Seminar at Spelman College, Atlanta, GA, October 9, 1997.

5.   John Hansen and James Burgess,  Trapping Intermediates During the Refolding of

the Bacterial Protein Glucose-6-Phosphate Dehydrogenase, presented at the 1997 Southeast Regional Meeting of the American Chemical Society, October 19-22, 1997.

6.   John E. Hansen, Michael Robbins, and Bryan Green,  Rescuing a Kinetically

Trapped Misfolded Protein, presented at the 1998 Southeast Regional Meeting of the American Chemical Society, November 4-7, 1998.

7.   John E. Hansen, Michael Robbins, Mary Kate McBrayer, and Yong Suh,  The Effect

of pH and Self-Association on the Unfolding Pathway of the Protein Azurin, presented at the 1999 Southeast Regional Meeting of the American Chemical Society, October 17-20.

8.   John E. Hansen, On the Rack will a Protein Divuldge its Secrets: A story of a blue

copper protein, Inorganic Chemistry Seminar Series at the University of Georgia, Athens, GA, March 27, 2000.

9.   John E. Hansen, Mad Hatters, Mad Cows, and Bad Protein, Sigma Xi Talk at the State

University of West Georgia, November 3, 2000.

 

Conference Participant

1.      Council on Undergraduate Research Institute Conference, North Carolina State

University, Ashville, NC, November 15-17, 1996.

2.      Laser Dynamic Laboratory - Laser Symposium, Georgia Institute Technology,

November 21, 1996.

3.      Symposium on Membrane Proteins, University of Wisconsin, Medical School,

Madison, WI, August 15 - 19, 1998.

4.      Laser Dynamic Laboratory - Laser Symposium, Georgia Institute Technology, January 21, 1999.

 

Review of Grant Proposals

3.      Panelist - Reviewer of NSF Instrument and Laboratory Improvement Grants, January

28 - 31, 1998.

4.      Panelist - Reviewer of NSF Course Curriculum and Laboratory Improvement Grants,

August 2-5, 1999.

 

Successful External Grant Proposals

 

1.   NSF Instrument and Laboratory Improvement Grant:          DUE-9751530

      Improvement of the Biochemistry Curriculum by Incorporation of Fluorescence

      and CD Spectroscopy

      Principle Investigator:  John Hansen

      Total Amount:         $127, 649

      Funding Period:      July 1, 1997  to  June 30 1999

2.   NSF Instrument and Laboratory Improvement Grant:          DUE-9751238

      Modernization of Undergraduate Curriculum through Laser Based Spectroscopies

      PI:  Farooq Khan

      Co-PIs:  John Hansen and Andrew Leavitt

      Total Amount:         $95, 730

      Funding Period:      July 1, 1997  to  June 30 1999

3.   NIH Research Initiatives at Minority Institutions

The Effects of Near-UV Radiation on Ocular Lens Crystallins in the Presence 

of Electrolytes

      PI: Lisa Hibbard, Dept. of Chemistry, Spelman College

      Co-PI:  John E. Hansen

      Total Amount:         $20, 000

      Funding Period:      September 1, 1998 to September 2000

 

4.   NSF-Major Research Instrumentation grant

      Acquisition of Liquid Chromatography and Mass Spectrometry to Foster Research-intensive Learning at the University of West Georgia

PI:  S. Swamy-Mruthinti

Co-PI: John E. Hansen and Farooq Khan

Total Amount:         $ 219,376

 Funding Period:     September 1, 2005 to August 31, 2008.

 

Unsuccessful External Grant Proposals

1.      Dreyfus Foundation Grant

Principle Investigator: John Hansen, Total Budget: $20,000.

2.      Research Corporation Cottrell Grant

Title: Spectroscopic Studies on the Mechanism of Chaperonin Facilitated Protein Folding, Principle Investigator: John Hansen, Total Budget:  $62,945.

3.   Petroleum Research Foundation Grant

Title: Spectroscopic Investigation of the Mechanism of Chaperonin-Facilitated Protein Folding, Principle Investigator: John E. Hansen, Total Budget:  $20,000.

4.   Research Corporation Cottrell Grant

      Title: A Kinetic Study of GroEL-assisted Refolding of Glucose-6-phosphate Dehydrogenase

      under Various Folding Conditions, Principle Investigator: John Hansen,  Total Budget:   

      $52,945.

5.   Petroleum Research Foundation Grant

      Title: A Kinetic Study of GroEL-assisted Refolding of Glucose-6-phosphate Dehydrogenase

      under Various Folding Condition, Principle Investigator: John E. Hansen, Total Budget:   

      $20,000.

6.   NIH AREA Grant

      Title: The Chaperone Function of Alpha-crystallin on Aquaporin-0

      PI:  S. Swamy-MruthintiCo-PI: John E. Hansen

      Total Budget: $100,000.

 

 

 

Successful Intramural Grant Proposals

 

1.   State University of West Georgia Student Research Assistant Program Award

      Amount:   $1650

      Awarded:  July 1, 1997

2.   State University of West Georgia Faculty Enhancement Reasearch Award

      Amount:  $1277

      Awarded:  July 1, 1997

3.   State University of West Georgia Student Research Assistant Program Award

      Amount:   $1650

      Awarded:  July 1, 1998

4.   State University of West Georgia Faculty Reasearch Grant

      Amount:  $ 2700

      Awarded:  July 1, 1998

5.   State University of West Georgia Student Research Assistant Program Award

      Amount:   $1650

      Awarded:  July 1, 1999

6.   State University of West Georgia Faculty Reasearch Grant

      Amount:  $ 1800

      Awarded:  July 1, 1999

7.   State University of West Georgia Faculty Reasearch Grant

      Amount:  $ 1800

      Awarded:  July 1, 2000

8.   State University of West Georgia Student Research Assistant Program Award

      Amount:   $1650

      Awarded:  July 1, 2000

9.  State University of West Georgia Faculty Reasearch Grant

      Amount:  $ 1500

      Awarded:  July 1, 2001

10.  State University of West Georgia Faculty Reasearch Grant

      Amount:  $ 1800

      Awarded:  July 1, 2002

 

11.  State University of West Georgia Student Research Assistant Program Award

      Amount:   $1500

      Awarded:  July 1, 2002

12.  State University of West Georgia Faculty Reasearch Grant

      Amount:  $ 1800

      Awarded:  July 1, 2003

13.  State University of West Georgia Student Research Assistant Program Award

      Amount:   $1500

      Awarded:  July 1, 2003

14.  State University of West Georgia Faculty Reasearch Grant

      Amount:  $ 1800

      Awarded:  July 1, 2004

15.  State University of West Georgia Student Research Assistant Program Award

      Amount:   $1500

      Awarded:  July 1, 2004

16.  State University of West Georgia Faculty Reasearch Grant

      Amount:  $ 1800

      Awarded:  July 1, 2005

17.  State University of West Georgia Student Research Assistant Program Award

      Amount:   $1500

      Awarded:  July 1, 2005

 

Matching Money provided by UWG

1.   Match for NSF-ILI grant DUE-9751530: $63, 825. 00

2.   Match for NSF-ILI grant DUE-9751238: $59, 865. 00

 

Donations of Materials and Equipment

1.   Plasmids that Express GroEL and GroES

Have worked out a legal agreement with E. I. du Pont de Nemours and Company for the material transfer of several different plasmids that over-expresses two proteins (GroEL and GroES).  These are proteins that I use in my research.

2.   The Bacterial Protein Azurin obtained from Pseudomonas aeruginosa

Acquired 1.6 grams of azurin obtained from Professor Russell Timkovich of the University of Alabama. This protein has a commercial value of $60/mg, bringing the total value for this amount of protein to over $100,000.  This protein is used in my research.

3.   Lasers

Have obtained two functioning lasers with power supplies and mode locker driver from Professor Graham Fleming of the University of California. The one laser is a mode-locked Nd:YAG laser capable of generating sub-nanosecond light pulses. The other laser is a continuous wave Nd:YAG laser.  Cost of the lasers when new was around $140,000.

 

 

Other Professional Experience

1.   Project Assistant - University of Wisconsin, School of Pharmacy,  8/80-8/81.

      Mass spectrometrist, operated and maintained  a quadrapole mass spectrometer.

2.   Academic Staff - University of Wisconsin, School of Pharmacy, 9/81-9/85.

      NMR spectroscopist for School of Pharmacy and Department of  Biochemistry.