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Descriptions GAPDH Ribosomal S6 LPL II PLC gamma Kelch/BTB Unknown ORF

Glyceraldehyde-3-phosphate dehydrogenase (partial)

Tallapoosa darter reference sequence (1026 nucleotides)

Amplified as one fragment:

   GAPDH exon-intron map


Genomic Sequence (exon, intron, PCR primer sites)
GAGTCCACAG GTGTATTCAC AACCATTGAG ARGGCCTCTG TACGTATCTA AATCATGTCT CAAACTCTTG AATAAAAGTA ATTAATACTG TACGACATGT GATGTGTAAT TGACCAAAAA GGACAAAGAT TAAATACTTT TTAACACATT ACAGGCTCAC TTGAGGGTGG TGCCAAGAGA GTCATCATCT CTGCTCCAAG CGCTGACGCT CCCATGTTCG TCATGGGCGT CAACCACGAG AAGTATGACA ACTCCCTCAC GGTTGTTAGG TAAGAACAAG GTTTTTTTGT TTTTGTGTTA AGTGGTTTGA AATTTAGTAT TTGAAAATGT GTTATTTAGT GTTTCACTCA TTGTACATCT GCTTCTGAAG TGTAAAAGAT AAAGGATGAA CGTTGTTGTG CTAGAATGTT TTAAGATGGT GTTTTCTTTT TTTCTTTCGA AAATCCGCAG TTAACTGATG CTGTTACTTT GCCTCTCCCA TAGCAACGCT TCCTGCACAA CCAACTGCCT GGCCCCCCTG GCCAAGGTCA TCAATGACAA CTTTGTCATC ATTGAAGGCC TGATGGTGAG GGTTTAAACA CGCCTGGGAA TAATCTGGCA GAAAGACACT CCGCAGCCCC TGAACCAGAT TACAAACAGC CCAAATCACA AACATAGGGG GGGCGGTTAG TCATATTTAA TCATCTTCTT CTCTTTGTCC ATCAGAGCAC AGTTCATGCC ATCACTGCCA CACAAAAGAC TGTAGACGGA CCCTCCGGCA AGCTGTGGAG GGACGGCCGT GGTGCCAGCC AGAACATCAT TCCCGCCTCT ACTGGTGCTG CCAAAGCTGT CGGCAAAGTC ATCCCTGAGC TCAATGGGTC AGTGCCAATA AATTTATTGC AGTATACTAT GTTATTGTAT GAACATGACT TGCCCTAAAA CCAGTGAATT TTCTTTTGTG ACCTTATCTA CTCTGTATTT CACAGCAAGC TGACCGGCAT GGCCTTCCGT GTCCCCACCC CCAACGTGTC CGTGGTTGAC CTGAC

cDNA Sequence (exon/exon/exon)

GAGTCCACAG GTGTATTCAC AACCATTGAG AAGGCCTCTG CTCACTTGAA GGGTGGTGCC AAGAGAGTCA TCATCTCTGC TCCAAGCGCT GACGCTCCCA TGTTCGTCAT GGGCGTCAAC CACGAGAAGT ATGACAACTC CCTCACGGTT GTTAGCAACG CTTCCTGCAC AACCAACTGC CTGGCCCCCC TGGCCAAGGT CATCAATGAC AACTTTGTCA TCATTGAAGG CCTGATGAGC ACAGTTCATG CCATCACTGC CACACAAAAG ACTGTAGACG GACCCTCCGG CAAGCTGTGG AGGGACGGCC GTGGTGCCAG CCAGAACATC ATTCCCGCCT CTACTGGTGC TGCCAAAGCT GTCGGCAAAG TCATCCCTGA GCTCAATGGC AAGCTGACCG GCATGGCCTT CCGTGTCCCC ACCCCCAACG TGTCCGTGGT TGACCTGAC

Protein Sequence (shading correlates to exons, codon spans adjoining exons)

ESTGVFTTIE KASAHLKGGA KRVIISAPSA DAPMFVMGVN HEKYDNSLTV VSNASCTTNC LAPLAKVIND NFVIIEGLMS TVHAITATQK TVDGPSGKLW RDGRGASQNI IPASTGAAKA VGKVIPELNG KLTGMAFRVP TPNVSVVDLT

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