About Genes Downloads Darterpedia News
Descriptions GAPDH Ribosomal S6 LPL II PLC gamma Kelch/BTB Unknown ORF

Lysophospholipase II (Acyl-protein thioesterase 2) (partial)

Tallapoosa darter reference sequence (2038 nucleotides total)

Amplified as three overlapping fragments:

    LPL exon-intron map
1) LPL II exons A to C (721 nucleotides)
2) LPL II exons C to E (687 nucleotides)
3) LPL II exons D to F (1000 nucleotides)


Genomic Sequence (exon, intron, PCR primer sites, overlapping primers site)
highlight colors indicate PCR primer pairs
CCCGATTCCC GTCACTCTCA ACATGAGGGC CATGATGCCC GCGTGGTGAG TTGGCGTGAT GAAACAGACA GCCTTTTAGC TCAACACTGG ATGCTATTGT TTCCCTGGAA ACCACAGCAG GCTCCCATAC CGGCATTGAC AATACAGCAA TTGTATATAT TGATTTCCTT AAACACGTGT CTCTTCATGT GTGCAGGTTT GACCTCATGG GTCTCAGCCC CGACTCCCAG GAGGACGAAT CTGGAATCAA GAAGGCAGCA GACAGCAGTG AGTTTATGTA AAAGTGTGCA CCGTTATATC CGTATTACCA TGATTATTGC TTCTCGTCCC TCTCTATGAT GATCGGCTTT TCACCCCATC TATAAGTATT GCCTTTCAGA ACATCTATAA ACACGTTTCC CTTTAGACGT GTGGATGAAC TAATTATTTC AGGCATACCC AGGCCTCTAG TTTTCGTCCT TGACCACTTA ACAGCCAAAA ATGTGTTTAG GATCTGAGGC TCCTCCTGGT GGTCACAGAC CACAACAACT ARAAGTAAAG CAAATGAAAC CAATCCTTAA AGTGACAGGG AAGTGTTTGT ATGGCAATCT GCACTATATG CTGTTTTCCT GATATGTTCA ATAACACATA TTTGTTCTTG CTTTCCCCCA GTTRAGGCCA TAATCGAACA TGAGGTCAAA AACGGGATCC CCCCGCACCG TATAATGCTC GGTGGCTTCT CTCAGGTTGG AATTCATATC TATATATCTA TATATGTTCT ATTTCACAAG AGTAATATCT GGAACTCAAT GTATATCATT ATCAGACAGC GTCAGCCGTG AAAGGCGTCA GAATTTGTTT TGATGAGTTA GGGTACAGTC TGTGAGAACT ATGTGGAGGC AATCCCAAAT CCCTTTGTTT
TCAGTGTTTT GAGAACGGCT CCTTTTAAAG AAAATATAAA TGAATGTATG TAATTAACAT CTCTATCTCT GTCTGCTTCC TGCCTTCCTT CTTAGGGTGG GGCCTTGTCC TTGTATACCG CCTTGACCTG CCAGCATCAG TTGGCTGGCA TTGTGGCCCT CAGTTGCTGG CTACCACTTC ACAGGAGTTT CCCATCGGTA CAGTCACAGT TAAATGCGTC TTCTGCCTCA TCAATTCAAA CTTCTTCAAA TGTCAAATGC TTTACTTTTG TCATAAAATA GTTCCAGTAA ACTTTCCCCT
AACCAATAGG GAAAACAATT CAGATTTAGC TTGCTTACTG TGGACATTGT TGTCTGTTTT GCAGGCGTCA AGCGGCAACA AGAACATCCC GATCCTGCAG TGCCACGGGG AGATGGACGT CATGATCCCA GTGCAGTTTG GTGCCATGAC GGCAGAGAAA CTCAAATCCA TAGTCAACCC TCAGATGATT ACCTTCAAAA CCTACCAAGG GCTCCCTCAC AGCTCCTGTC CTBAGGTAGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTTT GTTTGTGTGC ATGCGTGCGC
ATGCGTTTTT TATAAGATGC TTTTACAGTA GGGAGAGTAC GTAATTCAAT GTTAATTCTT ATTGCAACCA GCTCCAAACT ATTACTTCAT TCAGGTTTCC GTGTTTCAGT GTTGTTGGTA TATTTAACTA TAGAGTTTCA AACTCTGTAC AATCCTTCTG CTTGAACCAG GGCAGATTTG AACCAGATAT TTTCTGACAG ACTTTCTGAT TATTCAACCA GGGAGTGACA TTCCCTTAAT AGCCTTGAAA GTGCCCCACC TGCTGGTCAC CTGACATTGT ATACAGAAAA TACTTTCATT
TCCAGAAACC TGGCCTGCCG AAGTATACTC TCCCGCAACT CATACTTTCT AGGCTTATCT CATGGAAGTC TGTCTATCAA ACAAATAGTT CTCGTACTTT TGTAGACAAA CGGTTAGTAT TGTTGTGTTT TCCGTCAGCA ACAGTATAAT TTGTTTGTCC AAGGTACCAA CATCTACTTT GCTCTCTTGC AGGAGATGGC GGCTGTAAAG GAATTTATCG AGAAGTATTT GCCCCAAA 

cDNA Sequence (exon/exon/exon)

CCCGATTCCC GTCACTCTCA ACATGAGGGC CATGATGCCC GCGTGGTTTG ACCTCATGGG TCTCAGCCCC GACTCCCAGG AGGACGAATC TGGAATCAAG AAGGCAGCAG ACAGCATTAA GGCCATAATC GAACATGAGG TCAAAAACGG GATCCCCCCG CACCGTATAA TGCTCGGTGG CTTCTCTCAG GGTGGGGCCT TGTCCTTGTA TACCGCCTTG ACCTGCCAGC ATCAGTTGGC TGGCATTGTG GCCCTCAGTT GCTGGCTACC ACTTCACAGG AGTTTCCCAT CGGCGTCAAG CGGCAACAAG AACATCCCGA TCCTGCAGTG CCACGGGGAG ATGGACGTCA TGATCCCAGT GCAGTTTGGT GCCATGACGG CAGAGAAACT CAAATCCATA GTCAACCCTC AGATGATTAC CTTCAAAACC TACCAAGGGC TCCCTCACAG CTCCTGTCCT CAGGAGATGG CGGCTGTAAA GGAATTTATC GAGAAGTATT TGCCCCAAA

Protein Sequence (shading correlates to exons, codon spans adjoining exons)

PIPVTLNMRA MMPAWFDLMG LSPDSQEDES GIKKAADSIK AIIEHEVKNG IPPHRIMLGG FSQGGALSLY TALTCQHQLA GIVALSCWLP LHRSFPSASS GNKNIPILQC HGEMDVMIPV QFGAMTAEKL KSIVNPQMIT FKTYQGLPHS SCPQEMAAVK EFIEKYLPQ

Creative Commons License
This work is licensed under a Creative Commons Attribution 2.5 License.