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Descriptions GAPDH Ribosomal S6 LPL II PLC gamma Kelch/BTB Unknown ORF

1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1 (Phosphoinositide phospholipase C) (Phospholipase C-gamma-1) (partial)

Tallapoosa darter reference sequence (2853 nucleotides total)

Amplified as three overlapping fragments:

    PLC-gamma exon-intron map
1) PLC gamma 1 exons A to C (760 nucleotides)
2) PLC gamma 1 exons C to E (771 nucleotides)
3) PLC gamma 1 exons E to F (1478 nucleotides)


Genomic Sequence (exon, intron, PCR primer sites, overlapping primers site)
highlight colors indicate PCR primer pairs
CAGCAGCCAA GAAGAACTCA AGGAATGGGT GTTCAAGATC CGCGAGGTCA CCATGACCTC AGAGGCCAAG GTCAATATAC ATCTCTTGGC TCACAGAAAA TGCAAGCCAG GACTCATACC ATGCTCATGC CACACTGTGG GTTCAAAGAT CCAGTCATGA CTGTTGTTGG CCCTAAAATA CCCATAACCT GTATTTAGGA AGCACTGACT GAGTGTGCAT ATGAGCACAT ATAGTGTCAT GAATGCCATT CTGCAGACTC ACCTAGAAGC TGTCTTGTTT TGCTTTGTAC TGTTGAGGGG AGGAAAAAGC TTTAAAGTTT ATGTCCCTGT TTCCAGCTAG AGGAAGGGAA GATGATGGAG AGGAGGAAGA AGATTGCACT GGAACTGTCT GACCTGGTCA TCTACTGTAG ACCTGTGCCG TTTGATGAAG ACAGTAAGGA ATAGGCTTAT AATATGTGAA TATTCAGTTT AATACACACA GGAGCAGATA TGTGCCTGCT TTCATAGCTA TGACATTATA CAGGCACCAG TCCAATATTC AATACAGTTA GTTCAGGTCT GTCTGCTCTC TCTGCCTCCT GTAGAGATTG GCACAGAGCG GGCCTGTTTC CGGGACATGT CATCCTTCCC TGAGACCAAG GCAGAGAAGT ACGTCAACAA GATCAAAGGG AAGAAGTTCC TGCAGTACAA TCGACTGCAG CTGTCCAGGA TCTACCCCAG AGGCCAGAGA CTAGACTCTT CGAACTACGA CCCTCTGCCC ATGTGGCTCT GTGGGTCCCA ACTGGTTGCA CTTAACTTCC AGACAGCAGG TATCTAGCTT GTTGTCAGAG GAGATCAAAT GGCACACAAT ATCTTTTGTT TTTTTTTACT TTTCTCAACA ATTGTCTGGT TTTACCTTCC TCTTCCCTTC CCCACGTGCA GACAAGCCCA TGCAGATGAA CCAGGCTCTG TTTATGCTGA ACGGAAGGAG TGGCTACGTC CTTCAGCCGC CCATCATGAG GGATGACCAC TTTGATCCCT TCGACAGACT TACGCTACGA GGCCTTGAAC AAGTCACTTT ADAGATAGAG GTAAAGTGAA GAGAGGATTG GGCTGTGCAC ATGCGTGTGT GATGCCGTTG TGCAGAGGTT TTCAAACTGT AAGGTGCTCC TCCTAAGCTG GGTGTGGGAG AGTTGGAGAG AGGGGGCGCA AAGAGGCGTG AGACAGTTGT TTTAATTTTG TCGGGGGGGC ARTGTCAGTC TTTGACGTGT GTGTGTTTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTTTGAAACA AGTGAGTAAA CCGTATTGCT CTTTCCCTGT TCTGAAGGTT TTGGGTGCAC GGCACCTGCC CAAGCACGGA CGCGGCATAG TTTGTCCTTT GATCGAGATC GAGGTGTGTG GGGCAGACTA TGACAGTGCC AAGCAAAAAA CTGACTCAGA AGGTGAGCGC TGCTCTAAGT TTAACCATAG TTGGGTCTGC CAGTAAAGTA GTTACTGTTT ACTGGATACA TTACCCTCGT ATAATTTCCC TTTTGGTCCT TGAAAAACAG GAAGTTGTAC AAAGGTAAAA CATTGGACAC ACTTCTGACG GCTCTCATCA GATTTAAGCC AGAGAAGAAA CATACAAAAT GAGGTTAATG CTTTTGTTGC TTCATAATAT AGATTTGGTT TGCCACATAC AGTACAATGC CATTGTTTTT GCGCTTTTAC TCTCCTAAAA TTGTAATATA CTGTAGTCAA TAATAAGTAA TGTGAGTACA TTCTGCTTTG TTGAATTGTA CTTTCTAAAA TTTGTACTAA TCTCATCCTT TTTTGAAAGA TGGCGATTTT ATCATAGACA TTGGCAGCAT TTCTGTCACT ATATATTGAC CTCATTTGTT TTTTTTACCG TCACTCAGGG CAAAAATATG AATCACAATT CTTTTAGCTT AGAATTGATA TAATGATTTT CCGCCACAAT TTTTTCTCAC GAAGTGTAAT GTTTATTGCA CACATCAACC ATGACAAAAC AAAACAAATT GGCAGCACCA AACATCAATT TTTTTTGGCA CCTATAGCTC GTTGCGCATC ACCACAGAGA GGAGAGCATT GCAGTGCTTA AAACCTTTTA TACAGTCTGT TTTAAAATCT CACAGAAGAA AATAGTAGTT TGTGATCCAG TTGGCTCGTA AAATTAAATG AGAATCAAAG TCATAAAAAA AAGAAGGAAA AAAACGGGTG AATCGAGATC ACAATTTATC ACGCGGGTTT ACGGCACACC CAATAAAATA TTGAGCTTTC AATAAAGCAT TAAAGTTTGA TGTAGTATTG TTAGCCTGTT GAGGCATTCT CACCATTATC CATTATGGGG TGAGGATGTT TTTGCTTCTA AGGGCGGCTG ATTCTGAGGC TTCTGCAGTG GTAGGTTTAG TTTTTATTTG GCATCACTTC AAAATAATGA ATATGAATTT TCCTCTGCCG TTTGCACTTT TATTCATGGC GATCTRTTCA GCAATCGGGT TCCATCAGCC GAAGAAGCAA TGAGCCAAGG CAAAATAATT TTCCATAACC GACTCAGGTT TTTACAATTT TCTGTGTAAC ACAGTGATAC AATTAGCAAG TAGTAACCAT AATGTAAGTG CTGAAAGTTT TTTTTACACT GCTGTTACTG GGACAAAGTA ATGCTATTAC AGTTACGACA CCATTAGTGG ATACATATTG TACATTTCTC AATTATCTCT GTCCATCTTT CATTTGTCCT CTCCAGCTGA TAACGGCCTG AACCCCACGT GGCCCAGGAA GCCATTCCAG TTCACAGTAT GCAACTCTGC CTTCGCCTTC TTGCGCTTTG TGG 

cDNA Sequence (exon/exon/exon)

CAGCAGCCAA GAAGAACTCA AGGAATGGGT GTTCAAGATC CGCGAGGTCA CCATGACCTC AGAGGCCAAG CTAGAGGAAG GGAAGATGAT GGAGAGGAGG AAGAAGATTG CACTGGAACT GTCTGACCTG GTCATCTACT GTAGACCTGT GCCGTTTGAT GAAGACAAGA TTGGCACAGA GCGGGCCTGT TTCCGGGACA TGTCATCCTT CCCTGAGACC AAGGCAGAGA AGTACGTCAA CAAGATCAAA GGGAAGAAGT TCCTGCAGTA CAATCGACTG CAGCTGTCCA GGATCTACCC CAGAGGCCAG AGACTAGACT CTTCGAACTA CGACCCTCTG CCCATGTGGC TCTGTGGGTC CCAACTGGTT GCACTTAACT TCCAGACAGC AGACAAGCCC ATGCAGATGA ACCAGGCTCT GTTTATGCTG AACGGAAGGA GTGGCTACGT CCTTCAGCCG CCCATCATGA GGGATGACCA CTTTGATCCC TTCGACAGAC TTACGCTACG AGGCCTTGAA CAAGTCACTT TAGAGATAGA GGTTTTGGGT GCACGGCACC TGCCCAAGCA CGGACGCGGC ATAGTTTGTC CTTTGATCGA GATCGAGGTG TGTGGGGCAG ACTATGACAG TGCCAAGCAA AAAACTGACT CAGAAGCTGA TAACGGCCTG AACCCCACGT GGCCCAGGAA GCCATTCCAG TTCACAGTAT GCAACTCTGC CTTCGCCTTC TTGCGCTTTG TGG

Protein Sequence (shading correlates to exons, codon spans adjoining exons)

SSQEELKEWV FKIREVTMTS EAKLEEGKMM ERRKKIALEL SDLVIYCRPV PFDEDKIGTE RACFRDMSSF PETKAEKYVN KIKGKKFLQY NRLQLSRIYP RGQRLDSSNY DPLPMWLCGS QLVALNFQTA DKPMQMNQAL FMLNGRSGYV LQPPIMRDDH FDPFDRLTLR GLEQVTLEIE VLGARHLPKH GRGIVCPLIE IEVCGADYDS AKQKTDSEAD NGLNPTWPRK PFQFTVCNSA FAFLRFV

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