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Descriptions GAPDH Ribosomal S6 LPL II PLC gamma Kelch/BTB Unknown ORF

Ribosomal Protein S6 (partial)

Tallapoosa darter reference sequence (3182 nucleotides total)

Amplified as three overlapping fragments:

    S6 exon-intron map
1) S6 exons A to B (1630 nucleotides)
2) S6 exons B to C (982 nucleotides)
3) S6 exons C to D (821 nucleotides)


Genomic Sequence (exon, intron, PCR primer sites)
highlight colors indicate PCR primer pairs
CAAGGGTCAC TCCTGCTACC GTCCCCGCAG GACTGGTGAG CGCAAACGCA AGTCTGTCCG TGGTTGCATC GTTGATGCCA ATCTCAGCGT GCTCAACTTG GTCATCATCA AGAAAGGTAA TATGTTCGTT TTGGCTCGTT GACCATTATC TGAAAACAGA AACTTGGATA TGGAAACAAA CTATTGGGCA CTAAACCAAC AGATTATTCT CAAGGGGTAC ACAGCACAGG GTTATTAATA AATGTCTCTT AATTTTACCT GTAAATTATG TATGGCACAC CAGTAGACCC TTTCTGACTG ATCTATAGTC ATTAAGGGGG GACAGTGTGT TGCCAGAGTA ACTGTGGAGC TGCTTACCAG ATGCGACCAG GTCCAGGATC AGTGGATGGT TGAACTGCAT TGCTCTCTCA TTGGATCTCG TGTACACTAG TATCAGAATA ACTTATTTTA TAATATGACT TAATACAGCA GTATGTTGCT TTCTTTGCTA TTGGTGTAGT TTCCTGTTGA TGGGATGGTT AGCATGACAC TCATGAGTGT GATGTCCACT TGAGCTGTCA GTCTTACTCT AGTACCATTC AAATTTCATT TATATATCTT TTTCAACATC AGTCTGGCCT TGAGGGGGAC CTACATCATG GCVCATTGCA TTTCTGGCAT GCCTCTGTTT ACATTATTTT CCACCACGCA CACAGTGACA AATGCAACTG ACATGTAAGA AAGCACAGCC AATGATTTGC CATAAACTAT CACTAGCAAC GATGCTAAAG GCATTTATAA CTAAGCCAAA TGGTACTGTC ACTATTTTAG TGATTTCTAA GCAATCTGTT TCTTGCAGAT TGTCACGTTA CTATGCATTA GACTATGCTA ACTCTGACTG CTATAGAGCA GCTAACATTA ACTTAAGCTG TCTACATGAT GCTCCCGGCT AAGCTCCTAT AACTCACCAC AAATTTAGGA AACCAGAAAG GGCTAACTGA CAAGATAAGC ATTTTGGCTC GGTAGAAGGT TGATTTTTAT TTTATTTTTT TTAAAGTAAT GTTAAAATAT TTTCCATCCT TCTTACAATT TCCAGCCGCA GCCTGTCCCT CCCCCGGTAD GGTAATGTTA GCTCACAATG CCTTAGGTTT YTCMCACTTG AAACTTWTTG TTCATTAGAC CTGGAAGATG RGGTAATGTT ACMCCAACCT ATTTAAAAAA AACAAAAAAA AAAAACACCC ATTTGAATAG TAATGTCAGC ATTCGAATAG TATTGATTTT GTTTTACTGT TCGAATTGTT TTTGAGTTTT AGAATTTGTT TCCACAAAGT GTAGATAGTC GCCCTGTTTT TTTTGGTGCT TTGGGCTGCA GGCAGCTGCC GCTATTCACT GGGAAACGAC GCTATATTTT GTTTTAAGTG TTTGTTTTTT TTCTTCAAAT CATGCAAGTA CTTCAGATAC AGCATTGCGA CTTGTAAGTG AAATTGGTTT TTAATTATGA TTGCTTGACT CATCTCCTCA TGATCAGGTG AGAAGGACAT TCCCGGGCTT ACCGACAGCA CCGTCCCTCG CCGTCTTGGT CCCAAGAGGG CCAGCAAGAT CCGCAAGCTC TTCAACCTGG CTAAGGAGGA TGACGTTAGA CAGTATGTTG KGAGGAGACC CCTGACTAAA GAAGGTAAGT CTAGAGATAT ATTGTTAGAT TTACTTCACT GATCAAAGGG TTTTACTGGG TATGGATGTG CCTTTCATTG AAGCCTGAGA TTGTACGCCA CATGATGTCA GTTACTTCAA AATTAGATAA AATGGAGCTT GATATAAGGT TGTGGCTACT TCTTGGAACT ATATTGTAAA CACAACTTGT GTGTGTGTTA GATTTATACA TGTCATTACA TTTGTCAGTA TTCTCATCTT GTAATCCTCC ATATGGCTGA GGAGCAGCAA GAAAACATTT TCTAGTTCTA TAATTTAAGT TTCTTTGCTA GATACAACTT TAACAATATA ATTGTCTTTT ACTTAAATAT TTTTGATTTA TTTCCAACAA ATTAAAGTTT AAATTAATCC CACAATACAT CTTAGTTTAT ATATCACTGT TATGTGATCC AGTTACGCAG GTTAAAGTGT ATTGTAGATA GGCTGATGCT ATGTTAGTTG GCCAGTTTAC ATCATCAAAG AGCCTTTTCA AATTAGGGCT TGGGAGATAT ATCGATCGTG ATAAGAGGCT AGATCTCATC TTAGATTATG GATATCTTAT TTTGGTATAG TTGTCTTTTC ATGCTGCATT ATCGAATAGT GTTAAGTCAA ATCCTCAGAA CAGCTATTCT TGACAGTTGG CACCGTCTTT ACCACACCTA TCAATATTAA CTACATCCCC TCTCTTTGGT AGGCAAGAAG CCCAGAACTA AGGCTCCCAG GATCCAGAGA CTGGTGACAC CCCGTGTGCT GCAGCACAAG CGCCGCCGCA TCTCTCTCAA GAGACAGCGC ACCTTGAAGA ACAAGGAGGA GGCCTCTGAG TATGCCAAGC TGCTGGCCAA GAGGATGAAG GTAATGTTTT ATTGTTGTTC CTTATTACAG TCCTTAGGCA CTGAATTTTT CTCTTCAAAC CATATTCAGC TGAGGTGACC ACGAAACATT ACTTGGTGGT TTCAGATTAA TTAATAAATA GGTCAATTAT TAGATACATT TTTAGATAAT TTTGAAATTC AACACTATTT GCTGCTATAT TTTCCCTAAA AGGTCTAACC AGAGCAATAT TGAGGCCCTT TTTATATCAG TTGACATAAC TGTCTGTAAA TCTTAATTGA AGTTTAGATA TTACTGTACA GGAGTGCTGC ATAAAAGAAG TCGTCATTCT ATTTGTGTGC CGGCTTGTAT GGTGGTATCA TTCTTATAAG ATCACCTGCA AGTTGGACAT TTGTTGCAAT GCTTTTTTCA GCATAGGGAC ATATTTTTCA TTACACGTTA GTTCATGAAC TTAATGAACA ATTACCCTTA ACAGTTTACA GTTGCTGGCA TCAGAATAAA AGAATAAAAC TGTGTGTAGT AATTGTGTCA AGTGTGCAAT GTTGAGGAAT CCTATGTGGT ATGGGGGTAC TAACGTGTAT GGAAACGTAT GCTTTTTGAA TGTGTTAACA CTACTGACAC ATGTTTGTTT CCTGCAAACA GGAGGCCAAA GAGAAGCGCC AGGAACAGAT TGCCAAGAGG CGxxx

cDNA Sequence (exon/exon/exon)

CAAGGGTCAC TCCTGCTACC GTCCCCGCAG GACTGGTGAG CGCAAACGCA AGTCTGTCCG TGGTTGCATC GTTGATGCCA ATCTCAGCGT GCTCAACTTG GTCATCATCA AGAAAGGTGA GAAGGACATT CCCGGGCTTA CCGACAGCAC CGTCCCTCGC CGTCTTGGTC CCAAGAGGGC CAGCAAGATC CGCAAGCTCT TCAACCTGGC TAAGGAGGAT GACGTTAGAC AGTATGTTGT GAGGAGACCC CTGACTAAAG AAGGCAAGAA GCCCAGAACT AAGGCTCCCA GGATCCAGAG
ACTGGTGACA CCCCGTGTGC TGCAGCACAA GCGCCGCCGC ATCTCTCTCA AGAGACAGCG CACCTTGAAG AACAAGGAGG AGGCCTCTGA GTATGCCAAG CTGCTGGCCA AGAGGATGAA GGAGGCCAAA GAGAAGCGCC AGGAACAGAT TGCCAAGAGG CG

Protein Sequence (shading correlates to exons, codon spans adjoining exons)

KGHSCYRPRR TGERKRKSVR GCIVDANLSV LNLVIIKKGE KDIPGLTDST VPRRLGPKRA SKIRKLFNLA KEDDVRQYVV RRPLTKEGKK PRTKAPRIQR LVTPRVLQHK RRRISLKRQR TLKNKEEASE YAKLLAKRMK EAKEKRQEQI AKRR

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